| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus amylolyticus | TGGGCAAGACCACTCTCTC | AAGCCACATGTATAAGCGCAT | 58.63 | 58.42 | 287 | 149 |
100.00%
|
50.00%
|
| Primer 2 | Lactobacillus amylolyticus | TGGGCAAGACCACTCTCTCC | AAGCCACATGTATAAGCGCAT | 61.19 | 58.42 | 287 | 149 |
100.00%
|
50.00%
|
| Primer 3 | Lactobacillus amylolyticus | TGCGCTTTTGGTCGATGC | GCCAAGTGCTTTGCTGTCC | 59.44 | 60.01 | 204 | 146 |
100.00%
|
50.00%
|
| Primer 4 | Lactobacillus amylolyticus | AAGGTGGTGTGGGCAAGAC | AAGCCACATGTATAAGCGCAT | 60.15 | 58.42 | 296 | 141 |
100.00%
|
50.00%
|
| Primer 5 | Lactobacillus amylolyticus | AAAGGTGGTGTGGGCAAGAC | AAGCCACATGTATAAGCGCAT | 60.76 | 58.42 | 297 | 141 |
100.00%
|
50.00%
|
| Primer 6 | Lactobacillus amylolyticus | GGCATCGCAGATGCAAACC | GGCAGCATTAGCCCATTTACC | 60.23 | 59.66 | 261 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Lactobacillus amylolyticus | TTTTGCGGACGCCACAAC | TGCCACTGCACCAACCAT | 59.59 | 59.80 | 206 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Lactobacillus amylolyticus | ATTTTGCGGACGCCACAAC | TGCCACTGCACCAACCAT | 60.01 | 59.80 | 207 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Lactobacillus amylolyticus | TGGGCATTTCTGTCGGCA | TGCGCACCTCAGTCATGT | 59.57 | 59.26 | 171 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Lactobacillus amylolyticus | GGTTGATGTTGCCAGGCA | TCCGCAACATTAGCAGGCA | 58.22 | 60.00 | 234 | 138 |
100.00%
|
50.00%
|